Whole Goat Serum, 2mL

The total protein is measured using the Biuret procedure, with bovine albumin as the standard. The product gives a spectrum of normal serum precipitin arcs when tested by immunoelectrophoresis with anti-goat serum. The serum is delipidated, dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, filtered through a 0.22 µm filter, vialed and lyophilized. Whole Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Whole serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components. Whole serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Whole sera are also useful as controls for testing general and specific antibody purification methods. Product is whole goat serum and buffer salts. Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.