Cell Line Origin
Human Caucasian B lymphocyte
Cell Line Description
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
Application
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: human spleen. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 12352207
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- 93031001-1VL
- Temperature Control Device:
- Yes