General description
A Wnt-5a-derived, N-terminally butyloxycarbonyl- (Boc) protected, hexa-peptide that effectively antagonizes Wnt-5a-mediated cellular activities in A2058 and HTB63 melanoma cultures, including migration, invasion, calcium response, and MARCKS phosphorylation. Replacing the N-terminal Boc with a formyl modification results in a peptide termed Foxy5 that mimics, instead of antagonizes, Wnt-5a biological activity in a Frizzled-5- (Fzd-5/Frz-5) dependent manner. The peptide sequence is conserved in multiple species, including human, mouse, and rat, and Box5 is expected to exert its effect via direct FZD5 binding as is the case with Foxy5.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Sequence
t-Boc-NH-Met-Asp-Gly-Cys-Glu-Leu-CO₂H
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Jenei, V., et al. 2009. Proc. Natl. Acad. Sci. USA106, 19473.
Safholm, A., et al. 2006. J. Biol. Chem.281, 2740.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 766.88. Empirical Formula: C30H50N6O13S2. Quality Level: 100. Assay: ≥. 90% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, protect from light. color: white. solubility: water: 4 . mg/mL, DMSO: 50 . mg/mL. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 681673-10MG
