General description
A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP2)-induced actin assembly in Xenopus egg cytoplasmic extract (IC50 ~ 2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and prevent the activation of actin-related protein 2/3 (Arp2/3) complex by inhibiting the interaction between N-WASP and Arp2/3.
A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP2)-induced actin assembly in Xenopus egg cytoplasmic extract (IC50 ~2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and allosterically stabilize the autoinhibited conformation of N-WASP. It prevents the activation of actin-related protein 2/3 (Arp2/3) complex by blocking the interaction between N-WASP and Arp2/3.
Biochem/physiol Actions
Primary Target
Neural Wiskott Aldrich syndrome protein (N-WASP)
Target IC50: ~ 2 µM blocking phosphatidylinositol 4,5-bisphosphate (PIP2)-induced actin assembly in Xenopus egg cytoplasmic extract
Warning
Toxicity: Standard Handling (A)
Sequence
cyclo(LLys-DPhe-DPro-DPhe-LPhe-DPro-LGln)₂
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Other Notes
Peterson, J.R., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10624.
Suetsugu, S., et al. 2001. J. Biol. Chem.276, 33175.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51312609
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 681660-1MG
