General description

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Application

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Quality

Chromatographically homogeneous

Physical form

Crystals, white powder

Preparation Note

Working solution: Stock solution:

20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
A_{590 nm}: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 Â°C; when the solution is stored at -15 to -25 Â°C, it is stable for one year.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Molecular Weight: 408.63. Empirical Formula: C₁₄H₁₅BrClNO₆. description: 5-Bromo-4-chloro-3-indolyl-β. -D-galactopyranoside. Quality Level: 100. packaging: pkg of 1 . g (10745740001), pkg of 100 . mg (03117073001), pkg of 100 . mg (11680293001), pkg of 2.5 . g (10703729001), pkg of 250 . mg (10651745001). manufacturer/tradename: Roche. solubility: DMF: soluble, methanol: soluble. storage temp.: 2-8Â°C. SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O. InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1. InChI key: OPIFSICVWOWJMJ-AEOCFKNESA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.