General description
X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.
Application
X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the ApcΔ242 mutation in the tissue sample.
Warning
Toxicity: Irritant (B)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Quality Level: 100. form: solution. manufacturer/tradename: Novagen®. . storage condition: OK to freeze. technique(s): tissue culture: suitable. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): 188.6 °F - (refers to pure substance). Flash Point(C): 87 °C - (refers to pure substance).- UPC:
- 41116105
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 71077-3
