X-LINK IMMUNOPRECIPTIATION, 50

Immunoprecipitation (IP) is one of the most useful immunochemical techniques.IPs are routinely used to determinethe presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interactions with other proteins, nucleic acids and ligand s.IPs consist of four main steps: 1. Labeling of the antigen (Optional step) 2. Release of antigen by cell lysis 3. Formation of antibody-antigen complexes 4. Purification of the immune complexes. G-Biosciences Cross-Linking Immunoprecipitation kit contains all the reagents necessary to complete all aspects of immunoprecipitation, with the exception of labeling.Based on our Classical Immunoprecipitation kit , the Cross-Linking Immunoprecipitation kit incorporates the homobifunctional cross-linker DSS to covalently couple the antibody to the Immobilized Protein A/G resin . This additional cross-linking step prevents contamination of the purified antigen with antibody. The kit is designed for effective immunoprecipitations using <10µg antibody. The kit is suitable for 50 reactions using 10µl Immobilized Protein A/G Agarose. Features Eliminate Antibody Co-elutio n. Antibody is covalently coupled to the immobilized Protein A/G. Applications For traditonal immunoprecipitations without interference from antibody elutions.