X-TREMEGENE 9 DNA TRANSF. REAG. 1.0 ML (C15-1307-197)

General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Application

X-tremeGENE^™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE^™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:

• Expression of recombinant proteins for functional analysis.
• Physiological studies of metabolic pathways.
• Analysis of regulatory sequences using reporter gene assays.
• Gene expression assays.
• Cancer research studies.
• Target evaluation.

Features and Benefits

• Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
• Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
• Avoid time-consuming optimization procedures in commonly used cell lines.

Quality

Each lot of X-tremeGENE^™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE^™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

grade: for molecular biology. Quality Level: 100. form: liquid (aqueous solution). usage:  . mL (suitable for 165 transfections). packaging: pkg of 0.4 . mL (06365779001), pkg of 1.0 . mL (06365787001), pkg of 5 × 1.0 . mL (06365809001). manufacturer/tradename: Roche. technique(s): transfection: suitable. storage temp.: 2-8°C. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H319. Precautionary Statements: P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378. Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 334.4 °F. Flash Point(C): 168 °C.