General description
Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine. Y-27632 controls neutrophil migration and lung edema and may be a potential drug for treating acute lung injury.The utilization of Y-27632 dihydrochloride, inconjunction with Accutase or other enzyme solutions, during the passaging ofhuman embryonic stem (hES) cells has been demonstrated to enhance cell survivaland significantly improve cloning efficiency
Application
Y-27632 dihydrochloride has been used:
- as a medium supplement in pancreatic ductal adenocarcinoma organoid culture
- in the inhibition of Ras homolog gene family (Rho) kinase in mouse embryonic stem cells
- in the inhibition of Rho-associated protein kinase (ROCK) in human embryonic stem cells and human induced pluripotent stem cells (iPSCs)
- Y-27632 dihydrochloride has been used as a supplement in the E8 medium to promote in vitro differentiation of human pluripotent stem cells
Biochem/physiol Actions
Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.
Features and Benefits
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Legal Information
Sold under license from Mitsubishi Pharma Corporation
- UPC:
- 51161726
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- Y0503-5MG
- CAS:
- 129830-38-2
