Cell Line Origin
Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen
Cell Line Description
The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.
Immunogen
Ha-MuSV transformed syngeneic NRK cells
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask
Other Notes
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biological source: Rat x Rat Hybridoma. ImmunoDon: Osborne-Mendel rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116012
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 89111412-1VL