Yeast Nitrogen Base (YNB) with Ammonium Sulphate, 100 g

Yeast Nitrogen Base (YNB) w/ Ammonium Sulphate is used for the growth of all strains of Saccharomyces cerevisiae . This medium has been formulated after following the procedures of Wickerham and Burkholder (1, 2, 3). This is a well defined medium consisting of salts, vitamins and ammonium sulphate (nitrogen source). Addition of a carbon source is required for the growth of Sacharomyces cerevisiae . YNB w/ Ammonium Sulphate is formulated for investigation of yeast for their differential abilities in carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose. References: 1. Wickerham L. J., 1951, U.S. Dept. Agric. Tech. Bull. No. 1029 2. Wickerham L. J., 1946, J. Bacteriol., 52:293 3. Burkholder, P. R. 1943. Vitamin deficiencies in yeasts. Amer. J. Bot. 30:206-211 Appearance of Powder: Cream to yellow coloured, homogeneous, free flowing powder. Color and Clarity of prepared medium: Light yellow coloured, clear solution without any precipitate. Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours. Organisms (ATCC) Growth (plain) Growth (with Dextrose) Saccharomyces cerevisiae Poor-good good-luxuriant Storage and Shelf-life: Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.