Cell Line Description
The antibody is directed against the A epitope of the mouse L3T4 T cell antigen, the murine equivalent of CD4. Has been used in in vivo depleting and flow cytometry studies. Cambridge Enterprise Reference number AB16: www.enterprise.cam.ac.uk.
Immunogen
CBA mouse thymocytes
Culture Medium
RPMI 1640 + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
biological source: Rat x Rat Hybridoma. ImmunoDon: DA rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-L3T4, mouse. technique(s): cell culture | mammalian: suitable. isotype: IgG2b. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51294302
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87072282-1VL
