YUMM1.7D5 MOUSE MELANOMA CELL LINE (C005B-315634)

General description

Source:
The YUMM1.7D5 mouse melanoma cell line is a diploid clone derived from the YUMM1.7 cell line, which originated from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57/B1/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. The YUMM1.7D5 cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten and Cdkn2 (1).

The great promise of immune-based therapies in cancer and recent progress in successful application of these approaches has brought to the fore the necessity of immune-competent models to evaluate immune system responses to cancer cells. Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.

The YUMM1.7D5 mouse melanoma cell line is a diploid clone of the YUMM1.7 cell line, developed as an immunocompetent cell line that recapitulates genetic drivers found in a significant proportion of human melanomas (1). The diploid genotype of YUMM1.7D5 cells greatly enhances efficiency of CRISPR-based screens with higher frequency of biallelic knockout compared to tetraploid cells. YUMM1.7D5 is syngeneic with the immunocompetent C57/B1/6 mouse background, retaining genetic markers of the Braf/Pten mouse model in which melanoma tumors develop with short latency (2). The genetic tractability of the YUMM1.7D5 mouse melanoma cell line is especially suited for evaluation of potential immunotherapies and represents a valuable tool for tumor immunology and cancer biology.

References:
1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains >=1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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