Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Cell Line Description
The immunisation protocol involved repeated intranasal infection with human influenza virus and a single i.v. injection before fusion. Produces IgM which binds murine IgG2a of "a" allotype, Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.
Immunogen
A/PR/8/34 mouse pathogenic human influenza virus
Application
Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.
Culture Medium
RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: Sp2/0. Reactivity: Anti-IgG2a, mouse. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51202807
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93022519-1VL
