Cell Line Origin
Human Caucasian breast carcinoma
Cell Line Description
Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The cells are reported to express both the wildtype and variant oestrogen receptors, progesterone receptor and other steroid hormones.
Application
Hormone and tumourigenicity studies
ZR-75-1 has been used to determine the effects of cucurbitacin E (CuE) extracted from Ecballium elaterium A.Rich., Fam. Cucurbitaceae.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Culture Medium
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow very slowly, do not form a confluent monolayer and attach only lightly to the substrate. After subculture, it can take up to 7 days before cells re-attach. Detachment of cells can occur in PBS and trypsinisation can produce clumps of cells. To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. When subculturing, ensure the trypsin is completely removed by centrifuging the cells and resuspending them in fresh medium.
Other Notes
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biological source: human breast. growth mode: Adherent. karyotype: 2n : 46, modal no. 72. morphology: Epithelial. products: Not specified. receptors: Reported to express the oestrogen and progesterone receptors. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51201650
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87012601-1VL
