10—Capping Reaction Buffer (C007B-105117)

Product Descriptionten — Capping reaction buffer is a matching reaction buffer for vaccinia virus capping enzyme (gmp-m062) and mRNA cap 2 '-o methyltransferase (gmp-m072). one — The formula of capping reaction buffer is: 50mm Tris HCl (pH 8.0 at 25 °C), 5mm KCl, 1mm DTT, 1mm MgCl2.This product is produced with raw and auxiliary materials of medicinal specifications, and all kinds of pollution in the production process are strictly controlled. Product production and quality management procedures in line with GMP specifications ensure that the production process and all raw and auxiliary materials can be traced.Quality requirements project standard appearance Clear liquid Visible foreign matter Compliance with regulations PH value 7.5-8.5 Endonuclease residues Degradation of substrate shall not exceed 10% Exonuclease residues Degradation of substrate shall not exceed 10% RNase residue Degradation of substrate shall not exceed 10% Bacterial endotoxin content ‰¤1EU/ml Mycoplasma detection negative Heavy metal residues ‰¤10ppm Follow the following specifications1. ISO 9001:2015, certified facility€‚2. GMP appendix - cell therapy products State Drug Administration.3. general introduction to human gene therapy - Chinese Pharmacopoeia 2020, National Pharmacopoeia Committee.4. USP chapter <1043>, adjuvant materials for cell, gene, and tissue engineered products.5. USP chapter <92>, growth factors and cytokines used in cell therapy manufacturing.6. Ph. Eur. General chapter 5.2.12, raw materials of biological origin for the production of cell-based and gene therapy medical products.Product featuresVersatility: it is applicable to the enzymatic capping reaction of cap0 and cap1 RNA synthesis.Efficiency: this system can be used for the capping reaction of RNA produced by T7 RNA polymerase reaction, so that the capping reaction can be completed within one hour, the efficiency is close to 100%, and the correct direction can be ensured.Storage temperature-20±5 °C€‚Matters needing attention1. when using, please mix it upside down gently to avoid foaming, and use it after slight centrifugation. For the preparation and sub packaging of reaction solution, please use new (non RNase contaminated) gun heads, microtubes, etc. to ensure that all reagents are free of RNase contamination.2. to ensure effective reaction, please operate on ice.3. for your safety and health, please wear experimental clothes and disposable gloves. Specification: Pharmaceutical grade,10— Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C489098-SCI_40809cd29c0ac9df97091c41e9d28a68.pdf

UPC:

12161700

Condition:

New

Weight:

1.13 Ounces

HazmatClass:

No

WeightUOM:

LB

MPN:

C489098-5ml

Product Size:

5ml