1156QE/8

Cell Line Origin

Human embryonal carcinoma

Cell Line Description

A human embryonal carcinoma cell line established from a primary testicular tumour of a 22 year-old patient. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17

Culture Medium

DMEM (High Glucose) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). No sodium pyruvate.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO₂; 37°C. Cells need to be maintained at a high cell density.

Other Notes

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UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VL

MPN:

06072609-1VL

Temperature Control Device:

Yes