1306 HUMAN SKIN FIBROBLAST (C005B-235543)

Cell Line Origin

Human skin fibroblast

Cell Line Description

A HPRT- derivative of the SV40 transformed human fibroblast line GM0637. The cells were selected in 5μg/ml thioguanine and are useful for transfection experiments.

Application

Transfection studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: human skin. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41115711

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

VI

MPN:

90011887-1VL

Temperature Control Device:

Yes