174BR HUMAN SKIN FIBROBLAST (C005B-235545)

Cell Line Origin

Human skin fibroblast

Cell Line Description

A primary skin fibroblast established from an adult female with a cancer predisposition who also exhibited multiple primary tumours. The donor was the sister of 175BR (Sigma Catalogue number. 90011863).

Application

DNA repair studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: human skin. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

12352202

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

90011895-1VL

Temperature Control Device:

Yes