2' 7'-DICHLOROFLUORESCEIN DIACETATE BIOREAGENT SUITABLE FOR FLUORESCENCE >= 95% (HPLC)

General description

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

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UPC:

12161500

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

MPN:

35845-5G

CAS:

2044-85-1

Controlled Substances and Cyanide:

Yes