2-MERCAPTOETHANOL FOR MOLECULAR BIOLOGY FOR ELECTROPHORESIS SUITABLE FOR CELL CULTURE BIOREAGENT 99% (GC/TITRATION) (C005B-454344)

Application

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:

• as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
• to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
• to trypsinize mouse embryonic stem cells

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

41105300

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

MPN:

M3148-25ML

CAS:

60-24-2

Temperature Control Device:

Yes