3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.Application:3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectantto measure the levels of α-hemolysin toxinin imaging bound IgGs3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Specifications & Purity: This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.Application:3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectantto measure the levels of α-hemolysin toxinin imaging bound IgGs3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

MDL Number: MFCD00007748

Molecular Formula: C16H20N2

Molecular Weight: 240.35

EC Number: 259-364-6

PubChem CID: 41206

Isomeric SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51201829

Weight:

2.64 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

T755518-25ml

Country of Origin:

China

CAS:

54827-17-7

Product Size:

25ml

Harmonized Tariff Code:

2931.90.90

Hazard Statement Codes:

H302;H315;H319;H335

Precautionary Statement Codes:

P261;P264;P270;P271;P280;P321;P330;P302+P352;P304+P340;P305+P351+P338;P362+P364;P405;P403+P233;P501;P264+P265;P301+P317;P337+P317;P332+P317;P319