Sigma-Aldrich
4-NITROPHENOL SOLUTION 10MM
General description 4-Nitrophenol is a phenolic compound present as a chemical intermediate and breakdown product during the production of organophosphorus insecticide derivatives of parathion. It is an intermediate during the synthesis of
General description
4-Nitrophenol is a phenolic compound present as a chemical intermediate and breakdown product during the production of organophosphorus insecticide derivatives of parathion. It is an intermediate during the synthesis of paracetamol. 4-Nitrophenol is a chromogenic catalytic by product of the synthetic substrate 4-nitrophenyl phosphate. It is majorly used as a reference standard in alkaline phosphatase assay. 4-Nitrophenol is nonbiodegradable and its derivatives are used for herbicide and fungicide production.
Application
4-Nitrophenol solution has been used:
- in the generation of standard curve for quantifying alkaline phosphatase activity of mesenchymal stem cells
- bone marrow stromal cells (BMSCs)
- for quantifying β-hexosaminidase activity in HeLa cells
- as a standard for calorimetric determination of alkaline phosphatase activity at 405 nm
- for assessing the β-hexosamindase activity
Preparation Note
Prepared as a 1.39 mg/ml solution suitable for use as a calibrator in alkaline phosphatase assays (such as Bessey-Lowry-Brock method) using p-nitrophenol phosphate as a substrate.
- UPC:
- 51102816
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- Quantity:
- 100
- Unit of Measurement:
- ML
- MPN:
- N7660-100ML
- CAS:
- 100-02-7



