5-Fluoroorotic Acid Monohydrate, 1g

5-Fluoroorotic Acid monohydrate is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase. 5-Fluoroorotic Acid monohydrate (FOA) is used as a selective agent in yeast molecular genetics. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. It is also used in the selection of Ura cells in a population of Ura+ cells; and in the selection of orotidine-5’-phosphate decarboxylase (OMPdecase) mutants of Saccharomyces cerevisiae. FOA (0.1 mg/mL) has been used in the positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius. It is a noncompetitive inhibitor of dihydroorotase. FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in vitro and in vivo. FOA showed anti-tumor activity against transplanted tumors in rats and mice; a bacteriostatic effect in vitro against various microorganisms, particularly gram-negative bacteria; antimycotic activity against various types of mold.