Thermo Scientific 1-Step TMB ELISA Substrate Solutions 250 mL (C012B-031552)

Thermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution. TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1 mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range. Thermo Scientific Pierce 1-Step TMB ELISA Substrates detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax

UPC:

41142045

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

8

Unit of Measurement:

PK

WeightUOM:

LB

MPN:

34022