A2H HAMSTER CHINESE OVARY (C005B-234391)

Cell Line Origin

Hamster Chinese ovary

Cell Line Description

Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.

Application

Genetic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: hamster ovary. growth mode: Adherent. karyotype: 2n : 22. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

85011441-1VL

Temperature Control Device:

Yes