A673 HUMAN RHABDOMYOSARCOMA (C005B-234507)

Cell Line Origin

Human Ewing sarcoma

Cell Line Description

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.

The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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biological source: human muscle. growth mode: Adherent. karyotype: Near Diploid. morphology: Epithelial-like. products: Transforming growth factor (Tramnsforming Growth Factor (TGF)) - Ÿ. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

41181914

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

85111504-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes