A9MOUSE C3H/AN AREOLAR AND ADIPOSE TISSUE (C005B-234378)

Cell Line Origin

Mouse C3H/An areolar and adipose tissue

Cell Line Description

Derivative of L929 selected in azaguanine (3μg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

Application

Metabolic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: mouse adipose tissue. growth mode: Adherent. karyotype: Aneuploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

51131711

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

85011426-1VL

Temperature Control Device:

Yes