ACETYLCHOLINESTERASE FROM ELECTROPHORUS ELECTRICUS (ELECTRIC EEL) TYPE VI-S (C005B-087378)

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E^1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Preparation Note

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

UPC:

41141611

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

500

Unit of Measurement:

UN

MPN:

C3389-500UN

CAS:

9000-81-1