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Sigma-Aldrich

AF HDL AND LDL/VLDL ASSAY KIT (C005B-478015)

General description Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol

Description

General description

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable.

Suitability

Suitable for HDL and LDL/VLDL cholesterol detection in serum samples and for the evaluation of drugs on cholesterol metabolism.

Principle

The AF HDL and LDL/VLDL Assay Kit is based on an improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorometric assays.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

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Specifications
UPC:
12352202
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
Yes
Quantity:
1
Unit of Measurement:
KT
MPN:
MAK331-1KT
Temperature Control Device:
Yes
Catalog No. C005B-478015
Price: $1,145.11
List Price: $1,272.35
  • Fast shipping — 3-day lead time
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  • Shipping Lead-Time: 3-5 Days
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