Cell Line Origin
Rat Y3Ag1.2.3 x Rat DA spleen
Cell Line Description
The antibody is directed against peribacteroid material from Rhizobium-induced root nodules of Pisum sativum. The antigen is also present on plasma membranes and in the Golgi bodies, but not in endoplasmic reticulum (ER). The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.
Immunogen
Peribacteroid material followed by peribacteroid material depleted of bacterial lipopolysaccharide
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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biological source: Rat x Rat Hybridoma. ImmunoDon: DA rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-Peribacteroid membrane from Pisum sativum. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86030311-1VL
