AFRC MAC 65ANTI PERIBACTEROID MEMBRANE FROM PISU (C005B-234954)

Cell Line Origin

Rat Y3Ag1.2.3 x Rat LOU/Iap spleen

Cell Line Description

The antibody directed against peribacteroid membrane from Rhizobium-induced root nodules of Pisum sativum. The antigen is also found in plasma membranes and Golgi bodies, but not in endoplasmic reticulum ER. The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.

Immunogen

Peribacteroid material followed by peribacteroid material depleted of bacterial lipopolysaccharide

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

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biological source: Rat x Rat Hybridoma. ImmunoDon: Lou/Iap rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-Peribacteroid membrane from Pisum sativum. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

51283106

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

MPN:

86030313-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes