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Sigma-Aldrich

AFRC MAC 74 ANTI NITRATE REDUCTASE (C005B-235016)

Cell Line Origin Rat Y3Ag1.2.

Description

Cell Line Origin

Rat Y3Ag1.2.3 x Rat F344 spleen

Cell Line Description

The antibody is specific for NADH nitrite reductase and inhibits reduction of nitrate to nitrate using NADH electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) or methyl viologen. The antibody is directed against NADH-NR if mono and dicotyledons.

Immunogen

Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea oleraceal

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

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biological source: Rat x Rat Hybridoma. ImmunoDon: F344 rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-Nitrate Reductase. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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Specifications
UPC:
12352207
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
86112690-1VL
Temperature Control Device:
Yes
Controlled Substances and Cyanide:
Yes
Catalog No. C005B-235016
Price: $1,906.09
List Price: $2,117.88
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