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Sigma-Aldrich

ALBUMIN CHICKEN EGG GRADE VI (C005B-088436)

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein.

Description

General description

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.

Ovalbumin makes 75% of the total egg white protein.

Application

Ovalbumin has been used:

  • as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil
  • to induce allergic inflammation and atopic dermatitis in experimental mice
  • as a component in control mixture for validating tandem mass spectral analysis
  • as a model protein antigen to evaluate the in vivo efficacy of a chitosan/interleukin (IL)-12 adjuvant system

A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Biochem/physiol Actions

Albumin from chicken egg white serves as a natural adhesive.

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.

Physical form

Essentially salt free

Preparation Note

A further purification of A 5503 to reduce mannose content.

Specifications
UPC:
41181712
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
GR
MPN:
A2512-1G
CAS:
9006-59-1
Catalog No. C005B-088436
Price: $570.01
List Price: $633.34
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