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Sigma-Aldrich

AMIDO BLACK STAINING SOLUTION 2X ELECTROPHORESIS REAGENT

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane.

Description

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:

  • serum proteins present in the culture supernatant
  • cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
  • histones from chromatin samples

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

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Specifications
UPC:
51202200
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
Yes
Quantity:
1
Unit of Measurement:
EA
MPN:
A8181-1EA
CAS:
1064-48-8
Catalog No. C005B-005872
Price: $324.28
List Price: $360.31
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