Amylose Separoporer (Agarose)4B-CL, 10mL (C003B-357567)

Quantitative Analysis:  Protein extract from E. coli containing a plasmid that expresses a maltose-binding protein-β-galactosidase fusion protein (100,000 units) is passed over a 1 ml column at 4°C. The column is then washed with 10 column volumes of 20 mM Tris-HCl (pH 7.4), 0.2 M NaCl, 10 mM β-mercaptoethanol, 1 mM EDTA. The protein is eluted with above buffer plus 10 mM maltose. Electrophoresis on a 4-20% SDS-PAGE gel results in a single band. Regeneration:  Amylose Separopore can be regenerated by the following wash 1mmEDTA 3 - column volumes, 0.1% SDS - 3 column volumes, Water - 1 column volume, Column Buffer - 4 column volumes. Binding Capacity:   5-7 mg MBP- fusion protein/ml bed volume. Supplied As:  50% Suspension Storage Conditions:   20% Ethanol Storage Temperature:   4°C

UPC:

41105318

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

20181072-1