Annexin V Binding Buffer

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x10|6|/ml. The binding buffer contains an optimal concentration of calcium which is required for Annexin V binding to phosphotidylserine (PS) on the cell surface. Annexin V is a calcium dependent phospholipid binding protein that preferentially binds to negatively charged phospholipids including PS. Cells progressing through apoptosis can be monitored according to their Annexin V and propidium iodide staining pattern. Early apoptotic cells will bind Annexin V but are not sensitive to intracellular staining with propidium iodide (PI). As cells progress through apoptosis the integrity of the plasma membrane is lost, allowing PI to penetrate and label the cells with a strong yellow-red fluorescence.Annexin V Binding Buffer is a optimal formula containing 2.5 mM Calcium Chloride in HEPES buffered saline.Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml.Annexin V Binding Bufferis a buffer used with Annexin V reagents that is used for flow cytometry and immunofluorescence applications. Specification: 2.5 mM Calcium Chloride in HEPES buffered saline Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/A377916-SCI_9b29a665f454998b0598dbf176ff1045.pdf

UPC:

41116113

Condition:

New

Weight:

5.84 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

A377916-100ml

Product Size:

100ml

Precautionary Statement Codes:

P264