ANTI-AKT1/PKBALPHA ANTIBODY CLONE AW24 | 04-796

General description

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Specificity

This antibody recognizes Akt1/PKBα.

Immunogen

Epitope: C-terminus

Sythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα.

Application

Anti-Akt1 Antibody, clone AW24 detects level of Akt1/PKBα & has been published & validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Evaluated by western blot on RIPA lysates from human HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Akt1/PKBα in RIPA lysates from human HEK293 cells.

Target description

~60 kDa

Linkage

Replaces: 05-796

Physical form

Cultured supernantant containing 0.05% sodium azide.

Format: Purified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HEK293 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.