ANTI-ANKYRING MOUSE MAB (463)

General description

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of Ankyrin_G.

Recognizes the ~190 kDa ankyrin_G protein in brain extracts. Reacts with all spliced forms of ankyrin_G.

Immunogen

Human

a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin_G

Application

Frozen Sections (1-2 g/ml)
Immunoblotting (2-5 g/ml)
Immunofluorescence (1-2 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
Mouse or rat brain extract or unmyelinated axons

Other Notes

Ankyrin_G is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.

Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
Otto, E., et al. 1991. J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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UPC:

51262601

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

NB20-100UG

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes