Anti-Calcium Channel Cav3.1 Antibody (C15-1316-278)

Specificity

Recognizes Cav3.1. The epitope specific for Cav3.1 is not present in any other known proteins.

SPECIES REACTIVITIES:

The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.

Immunogen

Highly purified peptide corresponding to residues 1-22 of rat Cav3.1 (Accession O54898).

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Neuroinflammation & Pain

This Anti-Calcium Channel Cav3.1 Antibody is validated for use in WB for the detection of Calcium Channel Cav3.1.

Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_018896.3, NM_198376.1, NM_198377.1, NM_198378.1, NM_198379.1, NM_198380.1, NM_198382.1, NM_198383.1, NM_198384.1, NM_198385.1, NM_198386.1, NM_198387.1, NM_198388.1, NM_198396.1, NM_198397.1. UniProt accession no.: O43497. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: rat ... Cacna1G(29717). Hazard Statements: H412. Precautionary Statements: P273 - P501. Hazard Classifications: Aquatic Chronic 3. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3.