Anti-Capicua Antibody (C15-1317-594)

General description

Protein capicua homolog (CIC) , also known as Capicua Transcriptional Repressor, Capicua Homolog, and encoded by the gene CIC, is a transcriptional repressor which plays a role in development of the central nervous system (CNS). CIC is a new subfamily of the HMG-box superfamily and the gene′s expression is predominantly restricted to immature granule cells in the cerebellum, hippocampus and olfactory bulb in the CNS, and thus the gene is implicated in CNS development, in particular in granule cell development. CIC appears to be central to cerebellar development and ErbB signaling as well. Recent research has demonstrated that CIC is highly expressed in certain cancers including medulloblastomas, but also the loss of expression may serve as a biomarker for other cancer types such as oligodendrogliomas.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human Capicua near the N-terminal.

Application

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Capicua in human cerebral cortex tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Capicua Antibody is validated for use in Western Blotting for the detection of Capicua.

Quality

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Capicua in 10 µg of human cerebellum tissue lysate.

Target description

~160 kDa observed. Long isoform can be detected at ~250kDa with longer exposure, in some lysates (Kim, E., et al. (2013). Genea Dev. 27:590-595).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): ox (based on 100% sequence homology), hamster (based on 100% sequence homology), mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rat (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_055940. UniProt accession no.: Q96RK0. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CIC(23152). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.