ANTI-CD40 MOUSE MAB (EA-5)

General description

Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.

Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.

Recognizes the ~44 kDa CD40 protein in Raji cells.

Immunogen

transfected murine L cells expressing human CD40

Application

Flow cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Paraffin Sections (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.

Other Notes

FACS was performed on 5x10⁵ cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.

Fanslow, W.C., et al. 1992. J. Immunol.149, 655.
Law, C.L., et al. 1990. Leukemia4, 732.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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UPC:

41181912

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

MPN:

217590-100UG

Temperature Control Device:

Yes