ANTI-CDH1 (AB-2) MOUSE MAB (DH01)

General description

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.

Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.

Immunogen

Human

recombinant human Cdh1 protein

Application

Immunoblotting (1 g/ml)
Immunoprecipitation (2 g/mg protein lysate)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Analysis Note

Negative Control
Normal mouse serum

Positive Control
Junkat or LS174T cells

Other Notes

Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.

Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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UPC:

41181530

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

100

Unit of Measurement:

UG

MPN:

CC43-100UG

Temperature Control Device:

Yes