ANTI-CLATHRIN HEAVY CHAIN MOUSE MAB (X22)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.

Recognizes the ~180 kDa clathrin heavy chain protein.

This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.

Immunogen

Human

clathrin heavy chain purified from human brain

Application

Immunoblotting (1:500)

Immunofluorescence (1:1000, indirect)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Bauerfeind, R., et al. 1996. J. Neurocytol.25, 701.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.

Immunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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