Anti-CYP27B1 Antibody (C15-1317-465)

General description

25-hydroxyvitamin D-1 alpha hydroxylase (1αOHase; VD3 1A hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; or CYP27B1) is a mitochondrial enzyme that belongs to the cytochrome P450 family. It catalyzes the hydroxylation of 25-hydroxyvitamin D3 to form an active 1-alpha, 25-dihydroxyvitamin D3, in the kidneys and other tissues. 25-hydroxyvitamin D-1 alpha hydroxylase plays an important role in bone growth, calcium metabolism, and tissue differentiation. Previous studies have reported that mice deficient in 25-hydroxyvitamin D-1 alpha hydroxylase are affected by hypocalcemia, secondary hyperparathyroidism, retarded growth, and the skeletal abnormalities. In humans, defects in this enzyme causes pseudovitamin D deficiency rickets (vitamin D-dependent type 1A rickets), an autosomal recessive condition characterized by abnormal bone mineralization and impaired growth.

Immunogen

KLH-conjugated linear peptide corresponding to human CYP27B1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CYP27B1 in mouse and rat hippocampus tissue and in rat kidney tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.

Quality

Evaluated by Western Blot in serum free MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate.

Target description

~58 kDa observed. Uncharacterized bands may be observed at ~55 kDa, ~70 kDa, ~110 kDa, and ~115 kDa in some cell lysates. A doublet at ~58 kDa may be observed in some cell lysates (Kemmis, C. M., et al. (2006). J Nutr. 136(4):887-892.).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Serum free MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_000776. UniProt accession no.: O15528. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CYP27B1(1594). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.