ANTI-FAM83B CLONE 7D11 (C15-1888-386)

General description

Protein FAM83B (UniProt: Q5T0W9; also known as Family with Sequence Similarity 83, member B) is encoded by the FAM83B (also known as C6orf143) gene (Gene ID: 222584) in human. FAM83B is a member of an 8-member protein family (FAM83) that shares the highly conserved DUF1669. It is a proto-oncogene that functions in the epidermal growth factor (EGF) receptor signaling pathway. May activate both the EGFR itself and downstream RAS/MAPK and PI3K/AKT/TOR signaling cascades. It interacts with RAF1 leading to displacement of 14-3-3 proteins from RAF1, which then activates RAF1 within the RAS/MAPK signaling cascade. Higher levels of FAM83B are shown to increase PI3K/AKT signaling and cell proliferation. In EGFR-dependent breast cancer cells that express higher levels of FAM83B expression, ablation of FAM83B is shown to diminish p110 alpha and AKT membrane localization, which result in reduced AKT phosphorylation and cell proliferation. (Ref.: Cipriano, R., et al (2013). Oncotarget 4(5); 728-738; Cipriano, R., et al (2014). Oncogene 33(25); 3298-3306).

Specificity

Clone 7D11 specifically detects human FAM83B protein.

Immunogen

His-tagged full length human recombinant Protein FAM83B.

Application

Anti-FAM83B, clone 7D1, Cat. No. MABC1128, is a mouse monoclonal antibody that detects FAM83B and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected FAM83B in Western Blotting applications (Cipriano, R., et. al. (2014). Oncogene. 33(25):3298-306; Cipriano, R., et. al. (2012). J Clin Incest. 122(9):3197-210).

Immunocytochemistry Analysis: A representative lot detected FAM83B in Immunocytochemistry applications (Cipriano, R., et. al. (2012). J Clin Incest. 122(9):3197-210).

Immunoprecipitation Analysis: A representative lot detected FAM83B in Immunoprecipitation applications (Cipriano, R., et. al. (2014). Oncogene. 33(25):3298-306).

Quality

Evaluated by Western Blotting in HEK293T cells transfected with SN3-hFAM83B-HA.

Western Blotting Analysis: 1 µg/mL of this antibody detected FAM83B in HEK293T cells transfected with SN3-hFAM83B-HA.

Target description

~115 kDa observed; 114.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.