General description
Alpha-(1,3)-fucosyltransferase 3 (FucT-III; Galactoside 3(4)-L-fucosyltransferase) is highly expressed in the small intestine, colon, stomach, and kidney. It is a member of the glycosyltransferase family of proteins which are involved in a glycosylation pathway that attaches fucose to Lewis epitopes of target molecules. This type of post-translational modification allows modified (fucosylated) molecules to recognize specific target substrates. Fucosyltransferases may therefore play an important role in communication at the cell surface. FucT-III may play a role in blood group determination as it is involved in the expression of antigens such as Lewis B, sialy Lewis X, Vim-2, and Lewis A.
Specificity
No homology to Rat and Mouse was found.
This antibody recognizes FucT-III at the lumenal region.
Immunogen
Epitope: Lumenal region
KLH-conjugated linear peptide corresponding to the lumenal region of human FucT-III.
Application
Anti-FucT-III Antibody detects level of FucT-III & has been published & validated for use in Western Blotting.
Research Category
Cell Structure
Research Sub Category
Glycobiology
Western Blot Analysis: 1 µg/mL from a representative lot detected FucT-III on 10 µg of transfected lysate and did not detect the non transfected lysate.
Quality
Evaluated by Western Blot in human liver tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected FucT-III on 10 µg of human liver tissue lysate.
Target description
~50 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~50 kDa due to high glycosylation. (Christensen, L, et al. (2000). Glycobiology. 10(9):931-939.)
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human Liver Tissue Lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: Np_000140. UniProt accession no.: P21217. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... FUT3(2525). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABT115