ANTI-HUNK ANTIBODY (C005B-240595)

General description

Hormonally up-regulated neu tumor-associated kinase (HUNK) belongs to the protein kinase superfamily and is an SNF1-related serine/threonine kinase that has a unique COOH-terminal (C-terminal) part without homology to any known protein. This protein is highly expressed in the mammary gland, kidney, and central nervous system.

Specificity

Other homologies: Mouse (87% sequence homology) and Rat (85% sequence homology).

Immunogen

GST-tagged recombinant protein corresponding to human HUNK.

Application

Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected HUNK in human brain and human cerebellum tissues.

This Anti-HUNK Antibody is validated for use in WB, IH(P) for the detection of HUNK.

Quality

Evaluated by Western Blot in SK-BR3 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected HUNK on 10 µg of SK-BR3 cell lysate.

Target description

~70 kDa observed. The calculated molecular weight is 80 kDa, however HUNK has been shown as a ~70 kDa band in western blots. (Gardner, H.P., et al. (2000). Development. 127:4493-4509.)

Analysis Note

Control
SK-BR3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_055401. UniProt accession no.: P57058. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... HUNK(30811). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

Shipping Information:

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UPC:

41181936

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

100

Unit of Measurement:

UG

MPN:

ABC91

Temperature Control Device:

Yes