Specificity
Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991).
Application
Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation.
Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2μg/mL (Sato et al., 2005).
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
This Anti-Integrin α1 Antibody, clone FB12 is validated for use in ELISA, FC, IC, IH, IP, FUNC, WB for the detection of Integrin α1.
Target description
130 kDa
Physical form
Format: Purified
Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Analysis Note
Control
Microvascular endothelium
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41116126
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- MAB1973
- Product Size:
- 100/µG
