ANTI-INTEGRIN ALPHAVBETA5 ANTIBODY CLONE P1F6 AZIDE FREE

General description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Specificity

Reacts with the human integrin alphaVbeta5.

Immunogen

Human UCLA P3 cells (lung carcinoma)

Application

Detect Integrin αVβ5 using this Anti-Integrin αVβ5 Antibody, clone P1F6, azide free validated for use in FC, IH.

Inhibition of attachment of HT29 cells to vitronectin: >1:1,000

Inhibition of attachment of carcinoma and melanoma cells to vitronectin

Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)

Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only)

Immunoprecipitation

FACS

EIA

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Physical form

Format: Purified

Protein A purified

Purified immunoglobulin in 0.01M phosphate buffer, pH 7.1, 0.15M NaCl containing no preservatives.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Epithelium, keratinocytes

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Shipping Information:

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UPC:

12352200

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

MAB1961Z

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes